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Sharp drill and finish with a Dremel barrel sanding bit.

HTH

Mark

On 5/06/2013, at 8:48 AM, Jeremy Adler <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Heat the end of a metal tube in a Bunsen burnfer and then getnly push the end thru the base of the petri dish and then pull it out. The hole on one side has a clean smooth edge - stick on the glass coverslip. Cutters used for making holes in rubber bungs are effective.
> 
> 
> 
> Quoting jerry sedgewick <[log in to unmask]>:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Hello all,
>> 
>> I'm doing a demonstration for live cell imaging using an inverted, fluorescence microscope.  I'd like to do a fairly low tech method for the first demo.  My thought is to use a plastic culture dish, cut a square in the bottom, and then glue a cover slip to the bottom.  I will be filling it with pond water since I should find plenty of autofluorescent organisms.
>> 
>> I recall having tried to cut through a plastic culture dish and it wasn't easy.  I'm wondering if any of you have ever tried this and have had success at cutting through this sort of dish.  How did you do it while retaining smooth edges?
>> 
>> Any other means for accomplishing the same end would be appreciated for a project without a budget.
>> 
>> Thanks!
>> 
>> Jerry Sedgewick
>> 
> 
> 
> 
> Jeremy Adler
> IGP
> Rudbeckslaboratoriet
> Daghammersköljdsväg 20
> 751 85 Uppsala
> Sweden
> 
> 0046 (0)18 471 4607

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

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