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July 2013

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Subject:
Re: Neo scMOS problem
From:
simon walker <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 26 Jul 2013 08:39:41 +0000
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My understanding is that the Neo sCMOS sensor has two 11-bit (=2,048 grey level) analogue to digital converters, one with a high gain amplifier, one with a low gain amplifier.  In 16-bit readout mode the signal is a combined readout from the two amplifier/ADCs.  Presumably in rolling shutter mode you're just reading off from one of the amplifier/ADCs, choosing either the high or low gain option.  This is further confused by the chip being split into two halves with the top and bottom being read out independently, so presumably there are actually four amplier/ADCs in total.
Simon

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Lu Lei
Sent: 26 July 2013 08:57
To: [log in to unmask]
Subject: Neo scMOS problem

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Hi all,
We recently purchased a wide-field microscope system with a scMOS camera (Neo, 5.5 Megapixel, by Andor). We are having quite some annoying problems with this Neo scMOS camera. Though it is a 16-bit device, it does not have the dynamic range it should have in our system.
1)      In "Rolling" shutter mode, the intensity value that the camera could
generate is truncated at ~20K. In another word, the camera saturates at ~20K instead of 65535.
2)      In "Global" shutter mode, the camera saturates at ~57K-a little short of
65535.
The camera is controled by the latest MetaMorph. The cooling temperature of the camera seems also normal as displayed by MetaMorph (-30&#61616;C). We haven't received any explanation from Andor via local vendor yet. I am wondering what is configured incorrectly here.
Does anyone have similar experience of this camera or suggestion to share?
Thanks a lot.
Regards,
Lei Lu
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