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Seconding previous comments, I spoke with the local reps/engineers and they pointed out the switchover between amplifiers as well. Using Andor's own software (solis) you can select between all 3 modes (high gain, low gain, high dynamic range) and see what the effect is. Perhaps the local Andor people could get you demo access to solis and you could run some tests. That does not solve any metamorph related implementation questions though but might help answering which issues are truly camera related and which ones relate to the 3rd party driver.
Christian
On Friday, 26 July 2013 at 3:39 PM, David Baddeley wrote:
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> Following on from what others have said, it probably depends on how MetaMorph is reading the camera out (and what it is doing to the data afterwards - e.g. converting to photon counts etc ...). http://www.andor.com/learning-academy/dual-amplifier-dynamic-range-scmos-dynamic-range has some interesting info on how the Neo get's it's large dynamic range. Notable on that website is the following sentence "Due to the splicing together of the low and high gains, the transition region between them is not seamless but has been optimised as far as possible." To me this seems to suggest that if you're going to do deconvolution on the data (or any other form of quantitative data analysis), you might want to choose either the high or the low gain 11 bit mode, and live with the reduced dynamic range. It's quite possible that the MetaMorph engineers have made this choice when writing their camera code (It's certainly the safe default choice as it'll be sure not to
> generate artefacts whereas the combined mode might - knowing for sure would require a lot of testing, which might well be application specific).
>
> best wishes,
> David
>
> ________________________________
> From: Lu Lei <[log in to unmask] (mailto:[log in to unmask])>
> To: [log in to unmask] (mailto:[log in to unmask])
> Sent: Friday, 26 July 2013 3:56 AM
> Subject: Neo scMOS problem
>
>
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> Hi all,
> We recently purchased a wide-field microscope system with a scMOS camera
> (Neo, 5.5 Megapixel, by Andor). We are having quite some annoying problems
> with this Neo scMOS camera. Though it is a 16-bit device, it does not have the
> dynamic range it should have in our system.
> 1) In “Rolling” shutter mode, the intensity value that the camera could
> generate is truncated at ~20K. In another word, the camera saturates at ~20K
> instead of 65535.
> 2) In “Global” shutter mode, the camera saturates at ~57K—a little short of
> 65535.
> The camera is controled by the latest MetaMorph. The cooling temperature of
> the camera seems also normal as displayed by MetaMorph (-30C). We haven’t
> received any explanation from Andor via local vendor yet. I am wondering what
> is configured incorrectly here.
> Does anyone have similar experience of this camera or suggestion to share?
> Thanks a lot.
> Regards,
> Lei Lu
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> Lu Lei (Asst Prof) | School of Biological Sciences | Nanyang Technological
> University SBS-03n-18, 60 Nanyang Drive, Singapore 637551
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