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>Thanks everyone for the help with my antibody question. It's definitely
appreciated and the information I got will save me from some inevitable
headaches later down the road.
Cheers,
Scott

 Hi everyone,
> I was wondering if anyone has successfully used the Alexa Fluor 532
> secondary to view tyrosine hydroxylase in dopaminergic neurons. I'll be
> imaging it using a 543nm laser with a Nikon confocal microscope. Based on
> its
> excitation wavelength it seems to be an ideal match but I don't any
> experience with it.
>
> If not, can anyone comment on its anti-quenching properties? I'm currently
> using the Alexa Fluor 546 for the same purpose but it quenches almost
> unusably fast with every mounting media I've tried.
>
> Thanks for the help,
> Scott Wong
>
>