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Dear Andrew,
 
  You might find it really hard to use NIR emitting fluorophores no matter how 'bright' they are, specifically because your detector is of a LASM510 type. Segmented detectors, much more that regular PMT'es, are known to be not optimal for imaging in this spectrum range (check with Hamamatsu - source of all PMT'es for confocal microscopy). By the way this why the 'spectral scale on your machine ends at 710 nm as opposed to 800 nm with other spectral confocals.
But you probably noticed this already.
 
Best
 
Vladimir
 
Vladimir Zhukarev, Ph.D 
 Advanced Microscopy Consulting
 Life Science and Bio-Medical Applications
 
 'From Image-to Discovery!'
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 610.350.1510 www.from-image-to-discovery.com
--------- Original Message --------- Subject: Re: Fluorophores for 633nm laser
From: "Andrew Lindsay" <[log in to unmask]>
Date: 7/16/13 1:10 am
To: [log in to unmask]

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 Hi All,
 
 Thanks for all your replies and based on the advice I am leaning towards
 Alexa647 or DyLight649. To clarify I will be using the Meta detector on a
 Zeiss LSM510 to detect the far-red signal, and to further clarify I intend
 to use the secondary antibody in combination with GFP and Cy3 to do
 three-color immunofluorescence on mammalian cell lines.
 
 Andrew