***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Sunando, It depends on what assay you are performing, although our lab published a paper on Molecular and Cellular Biology where we applied a protocol to monitor the FN remodeling. You can find the paper through: Galectin Binding to Mgat5-Modified N-Glycans Regulates Fibronectin Matrix Remodeling in Tumor Cells <http://mcb.asm.org/content/26/8/3181.full> Mol Cell Biol.<http://www.ncbi.nlm.nih.gov/pubmed/?term=Galectin+Binding+to+Mgat5-Modified+N-Glycans+Regulates+Fibronectin+Matrix+Remodeling+in+Tumor+Cells#>2006 Apr;26(8):3181-93. Lagana A<http://www.ncbi.nlm.nih.gov/pubmed?term=Lagana%20A%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>, Goetz JG<http://www.ncbi.nlm.nih.gov/pubmed?term=Goetz%20JG%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>, Cheung P<http://www.ncbi.nlm.nih.gov/pubmed?term=Cheung%20P%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>, Raz A<http://www.ncbi.nlm.nih.gov/pubmed?term=Raz%20A%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>, Dennis JW<http://www.ncbi.nlm.nih.gov/pubmed?term=Dennis%20JW%5BAuthor%5D&cauthor=true&cauthor_uid=16581792>, Nabi IR<http://www.ncbi.nlm.nih.gov/pubmed?term=Nabi%20IR%5BAuthor%5D&cauthor=true&cauthor_uid=16581792> . Cheers, Ray On Mon, Aug 12, 2013 at 10:10 AM, sunando_imap <[log in to unmask]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > ***** > > Hello Everyone > We are planning to label fribronectin(Sigma) with Alexa 647. Our > starting amount is less than 5 micro gram of Fibronectin. > Do anyone of you have a working protocol to follow? > > Thanks in advance. > Sunando >