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October 2013

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Subject:
Re: Fura-2 filter question: how much light comes out of a microscope when using a Xenon arc lamp and good 340nm excitation filter?
From:
Arvydas Matiukas <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 28 Oct 2013 16:56:01 -0400
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George and Craig,
 
Below are my power measurements for a couple of widefield
Fura-2 systems.  I did not have UV power meter so to estimate
the power just used a visible range sensor at the shortest available
(400 nm) setting (which would provide somewhat underestimated values)
 
Polychrome V output: 83uW (340nm), 1570uW (380nm)
40x/340 objective output:  3.1uW (340nm), 110uW (380nm)
 
Xenon arc lamp output: 120uW (340/10, new filter), 450uW (380/10)
40x/340 objective output:  0.1uW (340nm), 6.7uW (380nm)
 
UV power transmission gets reduced as filter and liquid fiber get old.
It is very  important to use UV transparent objective otherwise
the transmitted UV power is reduced >10-fold.
 
I  hope this info helps. The above power levels are enough to provide
Ca imaging at ~100ms exposure. The power loss through the system 
is not as high as Craig reported  but my systems are widefield with fewer
optical elements.
 
Arvydas
~~~~~~~~~~~~~~~
 

 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
Department of Neurosci& Physiology
SUNY Upstate Medical University
766 Irving Ave., WH 3167
Syracuse, NY 13210
tel.: 315-464-7997
fax: 315-464-8014
email: [log in to unmask]
>>> Craig Brideau <[log in to unmask]> 10/26/2013 12:14 PM >>>
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UV power meters are not very common. Has anyone measured this? I'm doing
some confocal imaging with 380 nm and my loss through the system is a
factor of 2000-3000x (measured before scan head and after the objective.

Craig
On 2013-10-26 11:05 AM, "George McNamara" <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Dear Confocal listserv members,
>
> Fura-2 filter question: how much light comes out of a microscope when
> using a Xenon arc lamp and good 340nm excitation filter?
>
> How much for 380 nm filter?
>
> thanks in advance,
>
> George
>
> --
>
>
>
> George McNamara, Ph.D.
> Single Cells Analyst
> L.J.N. Cooper Lab
> University of Texas M.D. Anderson Cancer Center
> Houston, TX 77054
> Tattletales http://works.bepress.com/**gmcnamara/26/<http://works.bepress.com/gmcnamara/26/>
>

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