CONFOCALMICROSCOPY Archives

October 2013

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
mNeonGreen in a tandem fluorescent timer?
From:
Richard Mort <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 10 Oct 2013 13:00:08 +0100
Content-Type:
text/plain
Parts/Attachments:
text/plain (31 lines) 
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi, I'm wondering if anybody has experience with the above flurophore? 
In particular I'm interested in its fast maturation time - has anyone 
tried to measure this more accurately - they got it down to below 10mins 
in the original paper (Shaner et al Nature Methods 2013). Would it be a 
good substitute for sfGFP taking advantage of its yellow shifted 
emission and pairing it with Cerulean or mCherry?
Best
R

-- 
Dr Richard Mort
MRC Human Genetics Unit
MRC IGMM
University of Edinburgh
Western General Hospital
Crewe Road
Edinburgh.
EH4 2XU, UK

Tel: +44 (0)131 332 2471
Fax: +44 (0)131 467 8456


The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.

ATOM RSS1 RSS2