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From: Richard Cole [mailto:[log in to unmask]] 
Sent: Monday, October 07, 2013 10:15 AM
To: 'Confocal Microscopy List'
Subject: RE: Using a mirror for axial resolution testing

 

As part of a world-wide confocal microscope performance experiment, we (
<http://www.abrf.org/index.cfm/group.show/LightMicroscopyResearchGroup.54.ht
m>
http://www.abrf.org/index.cfm/group.show/LightMicroscopyResearchGroup.54.htm
) sent out test samples including sub-resolution beads for resolution
measurements.  We had consider using a front reflecting mirror and although
this is the method I use, the variability was higher than the beads.
Shameless plug: Cole et. al. International Test Results for Objective Lens
Quality, Resolution, Spectral Accuracy and Spectral Separation for Confocal
Laser Scanning Microscopes Microsc. Microanal. 19, 1-16, 2013
doi:10.1017/S1431927613013470

 

 

 

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core 
Wadsworth Center

 

Research Assistant Professor 
Dept. of Biomedical Sciences 
School of Public Health State University of New York 


P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax 

 

Email  <mailto:[log in to unmask]> [log in to unmask]

Website  <http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm