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Dear all,
Thanks a lot for the helpful advice! The problem was, indeed, wrong filters in the delivery, plus some dirt in the microscope.
Solved and running now.
All best,
Katja
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Prabhat, Prashant
Sent: Monday, November 04, 2013 11:28 PM
To: [log in to unmask]
Subject: Re: Olympus TIRF
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*** Commercial Response ***
Hi Neil,
Our popular TIRF filters have been in use by the microscopy community for several years and we have not come across issues with our filters, as you may have experienced. Therefore in a separate (off the Confocal List) email, I would like to connect with you to learn from your recent experience with Semrock filters for TIRF.
Sincerely,
Prashant
Prashant Prabhat, Ph.D.
Applications Scientist
Semrock, Inc., A Unit of IDEX Corporation
3625 Buffalo Road, Suite 6
Rochester, NY 14624
Direct: (585) 594-7064
Cell: (585) 520-8602
FAX: (585) 594-7095
[log in to unmask]
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Neil Anthony
Sent: Monday, November 04, 2013 8:49 AM
To: [log in to unmask]
Subject: Re: Olympus TIRF
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I agree with Paul. We just changed a Semrock for a Chroma filter cube set, which rectified a huge optical issue we were having that manifested as a large distinct region of non-TIR. There was no visual signs that the Semrock had anything wrong with it, and remounting (gently) didn't affect the problem.
The new Chroma line filter set works perfectly as far as I can tell.
see here:
http://chroma.com/product/complete-filter-sets/laser-applications/dual--TRF59904-EM--ET-488-561nm-laser-Dual-Band-set-for-TIRF-applications
Neil
On 10/31/2013 4:18 PM, Paul Paroutis wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Ekaterina,
>
> We have an Olympus CellTIRF system and have observed that choosing a
> good quality dichroic can make or break your system. In fact, we went
> through 3 'identical' dichroics before settling on one that gave the
> best images and the lowest background/light scatter. You may want to
> try a few different dichroics and see if that helps...
>
> Thanks,
> Paul
>
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