*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

One thing you might want to keep an eye on is that the Ri of the
immersion oil is temperature sensitive. I am not sure what that means
for STED at 37°. Maybe you can switch oils, to one that has 1.518 at 37.

Steffen


Am 28.03.2014 04:28, schrieb Adrian Smith:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Hi all,
> 
> We are considering applying for funds to upgrade our SP5 (c. 2008) to
> enable CW gated-STED.
> 
> Has anyone done this upgrade? How did it go?
> 
> One thing in particular that we are concerned about is that we routinely
> have the system at 37C for live work (it basically stays at 37 permanently)
> but the STED objective is apparently designed for room temperature...
> 
> Any comments appreciated,
> 
> Thanks,
> 
> Adrian Smith
> Centenary Institute, Sydney, AU
> 


-- 
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

Mail room:
Marchioninistr. 15, D-81377 München

Building location:
Marchioninistr. 27,  München-Großhadern