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March 2014

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Subject:
Re: Leica SP5 Upgrade to gated CW STED?
From:
Alison Dun <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 31 Mar 2014 14:54:45 +0100
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Hi,

We have a gated-STED on a Leica SP5 using a HyD and it works really well. We have done some CW-STED and the gated-STED is far better. I am not sure about the temperature as we have only done work on fixed samples so far. 

Cheers
Ali

Dr. Alison Dun
Technology and Facility Manager
Edinburgh Super Resolution Imaging Consortium (ESRIC)
Heriot-Watt University
Edinburgh	
EH14 4AS

Tel: +44 7977 518 581
@AliDun_esric

Register for our summer school at http://www.esric.org/summer-school.html





On 28 Mar 2014, at 18:06, Steffen Dietzel <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> One thing you might want to keep an eye on is that the Ri of the
> immersion oil is temperature sensitive. I am not sure what that means
> for STED at 37°. Maybe you can switch oils, to one that has 1.518 at 37.
> 
> Steffen
> 
> 
> Am 28.03.2014 04:28, schrieb Adrian Smith:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>> 
>> Hi all,
>> 
>> We are considering applying for funds to upgrade our SP5 (c. 2008) to
>> enable CW gated-STED.
>> 
>> Has anyone done this upgrade? How did it go?
>> 
>> One thing in particular that we are concerned about is that we routinely
>> have the system at 37C for live work (it basically stays at 37 permanently)
>> but the STED objective is apparently designed for room temperature...
>> 
>> Any comments appreciated,
>> 
>> Thanks,
>> 
>> Adrian Smith
>> Centenary Institute, Sydney, AU
>> 
> 
> 
> -- 
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
> 
> Mail room:
> Marchioninistr. 15, D-81377 München
> 
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