CONFOCALMICROSCOPY Archives

March 2014

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Subject:
Re: Alexa Fluor 405 Antibody
From:
Rob Palmer <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 28 Mar 2014 08:43:28 -0400
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

We use labeled primaries.  I love AFs in general, and we've tried this flavor more than once.  Poor coupling and/or weak photon flux (blue diode emission).  Your mileage may vary.

Robert J. Palmer Jr., Ph.D.
Microbial Receptors Section
Laboratory of Cell and Developmental Biology
Natl Inst Dental Craniofacial Res - Natl Insts Health
Bldg 30, Room 207
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-480-0112

On Mar 27, 2014, at 6:51 PM, Wong, Scott wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Hi everyone,
> I'm looking at getting some new antibodies and the Alexa Fluor 405
> secondary lines up well with the filters and laser lines I have available.
> Does anyone have experience with it, good or bad?
> Thanks for the help,
> Scott Wong

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