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One thought occurred to me on this thread: how much coverslip sealing is done on live cell preps vs. fixed-cell preps in your labs?
When I've done coverslip sealing (using melted household wax, mainly, though occasionally clear nail polish), it's been on live preps for immediate imaging, but also for non-curing mounting media on fixed cell preps for archiving for a day or so. Though when I have fixed cell preps and wish to archive, I've typically used a curing mountant.
Thoughts?
Jason
Jason A. Kilgore
Molecular Probes Tech Support
Thermo Fisher Scientific
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Littlejohn, George
Sent: Thursday, February 27, 2014 9:08 AM
To: [log in to unmask]
Subject: Re: Biological inert coverslip sealing
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Dear Antonio,
I can recommend Carolina Observation Gel (polydimethylsiloxane, and supplied by Blades Biological in the UK).
All the best,
George
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Dr. George Littlejohn
Halpin Laboratory,
School of Biosciences,
University of Exeter,
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EX4 4QD, UK
Telephone: +44 (0)1392725544
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On 27 Feb 2014, at 16:05, "Antonio J Pereira" <[log in to unmask]<mailto:[log in to unmask]>> wrote:
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Hi all,
For some purposes in live cell imaging we assemble a chamber with a coverslip on top of a slide.
We use two halves of another coverslip to act as spacers.
The issue is the sealing. We've been using VALAP (a kind of wax) that we melt and then use to seal all the way through the periphery of the coverslip.
It works, but it would be more convenient to have something that does not have to be heated, and that would harden just by contact with air.
A bit like the nail varnish used for fixed material ... but here we need something which is biologically inert.
Any suggestions? Thanks a lot.
Antonio
António Pereira
Chromosome Instability and Dynamics Group, Maiato lab
IBMC - Institute for Molecular and Cell Biology
Porto- Portugal
António Pereira
IBMC - Instituto de Biologia Molecular e Celular
Grupo Instabilidade e Dinâmica Cromossómica
Rua do Campo Alegre, 823
4150-180 Porto
Portugal
+351 22 607 49 00 (ext# 1620)
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