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Hi everyone,
A user is planning an experiment where spectral separation of mCherry and
tdTomato in the same mouse tissue will be needed (single photon). I know
that their peak emissions should be ~25 nm apart so this seems very doable
on my LSM 710, but I wanted to check...has anyone actually spectrally
unmixed mCherry and tdTomato successfully?
Thanks,
Esteban