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We decided to give the Thorlabs high sensitivity CMOS camera as USB3 version a try (http://www.thorlabs.com/thorproduct.cfm?partnumber=DCC3240M). Mainly because of the mentioned high QE, higher resolution and high frame rate, as well as a trigger support.

Thanks for all the recommendations on this topic!

Jens


On Mar 31, 2014, at 3:09 PM, David Baddeley <[log in to unmask]> wrote:

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> 
> If you're willing to push the budget to ~1100, we've been very happy with the Thorlabs high sensitivity CMOS (DCC1240M  http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4024). The read noise is a bit high (estimated ~ 20-30 electrons), and somewhat non-uniform, but the QE is very good - we've even managed to get single molecule signals out of it.
> 
> cheers,
> David
> On Monday, 31 March 2014 2:41 PM, "Knecht, David" <[log in to unmask]> wrote:
> 
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> 
> Does this adapter support binning with any of the cameras?  We find binning important for fluorescence and the adapter with the Sony XCD700 does not seem to support binning.  Dave
> 
> On Mar 31, 2014, at 12:27 PM, Johannes Schindelin <[log in to unmask]<mailto:[log in to unmask]>> wrote:
> 
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> 
> Hi Jens,
> 
> On Mon, 31 Mar 2014, Jens-Bernhard Bosse wrote:
> 
> I am looking for a budget camera (under 1K) for a microinjection/routine
> fluorescence rig. I will do the microinjection in phase contrast and
> need some fluorescence capability to check for microinjected fluorescent
> beads and fluorescent cells. I am not too sure if CMOS or CCD are better
> for the job. Port-wise USB, Firewire or GigE would be all fine. A higher
> frame rate capability (e.g. 25 fps) would be great as well as a
> resolution around one megapixel. Ideally it should be supported by
> either Matlab or Micromanager.
> 
> We dabbled with TheImagingSource cameras (supported by Micro-Manager, and
> through Micro-Manager in Matlab, of course). The quality was decent, but I
> would recommend the Firewire version because our USB model seemed to have
> some problems with heat when switched on for eight hours or so. That might
> be fixed in the meantime, though.
> 
> Another budget option might be IIDC machine vision cameras (supported by
> Micro-Manager: http://www.micro-manager.org/wiki/Dc1394 -- limited Windows
> support).
> 
> Having said that, in our project we were lucky to get ahold of an Orca
> that was not currently in use, and the quality of the images is noticably
> better. So we currently use neither of the cameras mentioned above.
> 
> Ciao,
> Johannes
> 
> David Knecht, Ph.D.
> Professor and Head of Core Microscopy Facility
> Department of Molecular and Cell Biology
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)