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I was looking at mitotic cells, that's why you see the punctate signal, since it localizes at kinetochores and mitotic spindle at this stage.
Good luck with your stainings!

On Apr 9, 2014, at 10:06 PM, PengKe wrote:

> Thanks a lot Martin. I will order this one and give it a try. 
> I had a look at your figures. The punctate signals in the cytoplasm seems to be a little bit less than I expected since Dynein is the major retro-grade motor protein. But I had no experience with Dynein and cytoskeleton was not really relevant for me in the past so my expectation has no solid ground. I think I will try and see.
> Thanks again.
> Aro 
> 
>> Date: Wed, 9 Apr 2014 18:16:18 +0100
>> From: [log in to unmask]
>> Subject: Re: Specific antibody of Dynein heavy chain in Immunofluorescence analysis
>> To: [log in to unmask]
>> 
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>> To join, leave or search the confocal microscopy listserv, go to:
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>> Post images on http://www.imgur.com and include the link in your posting.
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>> 
>> Aro,
>> this one was quite good for me:
>> Dynein Heavy Chain 1 rabbit polyclonal antibody: HPA003742, Sigma.
>> I was using methanol fixation and diluting it 1:50 - 1:100.
>> You can take a look at the images in this paper:
>> http://www.ncbi.nlm.nih.gov/pubmed/20427577
>> Regards,
>> Marin
>> 
>> On Apr 9, 2014, at 5:02 PM, PengKe wrote:
>> 
>>> Hello Everybody:
>>> Can somebody recommend a specific antibody of Dynein heavy chain (or other subunits but preferentially the heavy chain) for Immunofluorescence analysis? 
>>> I have been looking at the available options from several (prestigious) companies but feel that with this large number of 'promising' options it is not very easy to choose. 
>>> I will be very grateful if someone can share some experience on this.
>>> Many thanks in advance.
>>> Regards,
>>> Aro 		 	   		  
>