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 This is off-topic, but what other methods have people used to introduce
controlled astigmatism? I've used a moderate focal-length cylindrical lens
directly in front of the camera for 3D localization, but our field of view
wasn't very big, and I never carefully checked that the astigmatism was
uniform across a large FOV.

 Would a flat, tilted piece of glass in a non-infinity space be a better
way to achieve the same goal? If it works, you could tune the magnitude of
astigmatism by adjusting the tilt angle, and I'm guessing the astigmatism
would be more uniform across the field. Is this a bad idea, an old idea, or
something worth trying?


On Wed, Apr 23, 2014 at 1:38 PM, Dan Focht <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> Zulin
>
> What shape are you able to acquire the 1m Cyl lens.
> Tell me more about what is available and I will tell you what we can do
> with it.
>
> Dan
>
>
>
>
>
> On Apr 22, 2014, at 11:40 PM, Zulin Yu wrote:
>
> *****
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> *****
>
> Dear All,
>   We are planning to add cylindrical lenses with 10m focal length to our
> microscopy to do 3D STORM. Unfortunately, I am having problem to find any
> round cylindrical lens (1 inch) with such a long FL. As far as I know, CVI
> is the
> only company making 10 m cylindrical lens, but they are not willing/able
> to cut
> their lenses to round shape (1 inch). It will be really helpful if anyone
> could tell me
> which company can make these lenses. Thanks a lot, Zulin
>
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: (724)282-7145
> F: (724)282-0745
> [log in to unmask]
>