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The Invivo scientific we have also has the sensor at the sample.
Thanks,
Prabhakar
--------------------------------
From: Oliver Biehlmaier <mailto:[log in to unmask]>
Sent: 5/16/2014 9:03 AM
To: [log in to unmask] <mailto:[log in to unmask]>
Subject: Re: stagetop incubators
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Hi Jeff,
You are right. With the Okolab setup you can measure directly in your sample or “slightly indirectly” bei measuring in a reference dish in the stage top-CO2-incubator. All other incubators in our facility feature the other setup with the sensor in the cage (but not in the stage top incubator) somewhere close to the sample
Cheers,
Oliver
Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
Phone: +41 61 267 20 73 | Email: [log in to unmask] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch
On 15 May 2014, at 14:26, Jeff Reece <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Oliver,
>
> Could you elaborate on the statement:
> “The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample.”
> This is counterintuitive to me, as I would think one would monitor the sample if one is trying to control its temp.
>
> Cheers,
> Jeff
>
> From: Oliver Biehlmaier
> Sent: Thursday, May 15, 2014 3:12 AM
> To: [log in to unmask]
> Subject: Re: stagetop incubators
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Jeff,
>
> We have several types of incubators in our facility: PeCon (comes with Zeiss systems), LIS (comes with Leica systems, at least in CH), OkoLab. All of these systems are cage incubators and offer stable and reliable temp. control. PeCon and OkoLab cages are more closely fitted “around the stage and the core optical components” whereas the LIS ones are more bulky and put more or less a big incubator box around the entire stand. Since a few years LIS offers cages where 1/3 or 2/3 of the cage (you can choose) can be taken off easily. Especially with the 2/3 solution it is pretty easy to service most parts of the system without deinstalling the entire incubator. This is a big advantage.
> The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample.
>
> Concerning CO2, we use additional stage top incubation inserts on all the systems in order to provide very precise and fast onset CO2 levels in a rather small volume that is easier to control. All brands control the gas flow and mixing very nicely. However, the humidifiers turn out to be the most critical point in all gas mixers. We prefer the ones that are using more or less normal red or blue cap bottles (OkoLab PeCon) as they can be much easier refilled, especially by users. Other brands like LIS offer slim, long humidifier bottles that are positioned inside the incubator and thus have the same temp. which is very good for temp. stability. However, refilling the water can be tedious and you should be prepared that you or at least your users break at least on bottle…
> Since 2013 IBIDI also offers a nice gas mixer that can be added to any other incubation chamber.
>
> Concerning prices you will have to ask for offers. Prices change continuously and it is hard to tell who is cheapest or most expensive at a time…
>
> I hope that helps!
> Cheers,
> Oliver
>
>
>
>
> Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
> Phone: +41 61 267 20 73 | Email: [log in to unmask] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch
>
>
>
>
> On 15 May 2014, at 03:15, Jeff Spector <[log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Greetings,
>> I am looking in to purchasing a stage top incubator for our spinning
>> disk system so we can do live cell imaging. I've looked a lot at the Tokai
>> Hit setup but have also heard good things about stuff from OKO lab. Do you
>> microscopy experts have any suggestions/advice, or opinions about what
>> system we should use?
>>
>> thanks!
>> -Jeff
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