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May 2014

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Subject:
From:
Loralei Dewe <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 16 May 2014 12:21:34 -0700
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*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Neil,

The optiMOS is a very nice camera, it does have the rolling shutter, QE is
about 55%  but it still runs the 100 fps at full res and is compatible with
many of the current software packages ;-)

The sCMOS cameras that use the USB 3.0 are not going to run as fast with
the USB restriction. The pco cameras run at 40 fps but I believe the
Hamamatsu cameras only do 30 fps. Correct me if I am mistaken..

Loralei


On Fri, May 16, 2014 at 7:26 AM, Neil Anthony <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Emmanuel, I'm not sure if it's one of the latest options or not, but has
> anybody looked at the optiMOS from QImaging?
>
> It has a CIS1920F sCMOS sensor, and everything else looks as good as the
> others but with a reduced sensor size (1920x1080).  It's less that 10k, so
> it's considerably cheaper.  They don't mention the shutter type, but it has
> to be a rolling shutter, right?
>
> Also, there's the Flash 4.0 LT.  I think it's closer to 12k instead of the
> 16-20k of the fastest and global shutter versions.  It's USB 3.0 which
> makes life a little easier, and it's got a better QE and less noise than
> the cheaper version of the Zyla 5.5 (which does offer 30% more real estate;
> 2561x2160 vs. 2048x2048 both with 6.5µm pixels).
>
> Does anybody know of a webpage/document out there that has all the details
> and prices in one place?  It would be great to condense all these things.
>
> Neil
>
>
>
> On 4/19/2014 12:22 PM, Emmanuel Levy wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear All,
>>
>> We intend to buy a spinning disk confocal microscope and we are therefore
>> looking for the best camera to suit our need.
>>
>> The main use will be imaging of fluorescently tagged proteins expressed in
>> live cells.
>>
>> One thing is certain, we will buy a sCMOS, but we are unsure of which one
>> is best.
>>
>> For a while, it is my understanding that the Hamamatsu Flash4 V2 was the
>> best - but couple of month ago the same chip has been used in the Zyla 4.2
>> (Andor) and in the PCO Edge 4.2.
>>
>> All of them exhibit highly similar characteristics, with QE ~70%, low read
>> noise, 100fps with full chip, etc.
>>
>> I guess the only way to see what exactly are the advantages/drawbacks
>> would
>> be to use them in the same setup and compare them side by side.
>> Unfortunately I cannot do that and therefore I'd be happy to hear about
>> any
>> experience you have.
>>
>> I insist that only the late models should be compared (released in the
>> fall
>> of 2013 I believe). If you have had a bad experience with previous models
>> (e.g., Andor Neo), it does not apply here.
>>
>> Thanks in advance,
>>
>> Emmanuel
>>
>>

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