*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Iım looping my friend, Lauren, in on this conversation because I know her
lab at University of Wisconsin has developed microfluidic devices for this
purpose.
Dr Pamela A. Young
| Light and Optical Microscopist
Australian Centre for Microscopy & Microanalysis
THE UNIVERSITY OF SYDNEY
Rm 116A, Madsen Building F09 | The University of Sydney | NSW | 2006 |
Australia
T +61 2 9351 7527 | F +61 2 9351 7682
E [log in to unmask] | W http://sydney.edu.au/acmm
Incorporating:
Australian Microscopy & Microanalysis Research Facility (AMMRF) | W
http://www.ammrf.org.au <http://www.ammrf.org.au/>
ARC Centre of Excellence for Design in Light Metals | W
http://www.arclightmetals.org.au <http://www.arclightmetals.org.au/>
CRICOS 00026A
This email plus any attachments to it are confidential. Any unauthorised
use is strictly prohibited. If you receive this email in error, please
delete it and any attachments.
On 5/06/2014 11:23 pm, "Cameron Nowell" <[log in to unmask]> wrote:
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Hi Shalin,
>
>Like Guy said we have done this at the Monash Live Cell Course. Normally
>we have used younger embryos but for older ones we have had good luck
>with CyGel. In the past i have done other imaging with older (96hpf)
>embryos and have done it just with low melt temp agarose. Its good for
>about 24 hours of imaging. If you need more then you have to chop away
>part around the tail end to allow the embryo to grow.
>
>I also know of a group here in Melbourne that are building lab on a chip
>type microfuidic devices to hold embryos (2-30hpf) in place. They are
>really amazing little things and let you load up a heap of embroys with
>each one locking into place using micro fluidics. These can then be
>imaged without a problem
>
>Drop me a message off list and i can put you in touch with them.
>
>Cheers
>
>Cam
>
>
>
>Cameron J. Nowell
>Research Facilities Manager
>
>Monash Institute of Pharmaceutical Sciences
>Monash University
>399 Royal Parade
>(Mail address: 381 Royal Parade)
>Parkville, VIC, 3052
>Australia
>
>Email: [log in to unmask]
>Mobile: +61 422882700
>Office: +61 9903 9587
>
>LinkedIn: Profile
>Research Gate: Profile
>
>
>________________________________________
>From: Confocal Microscopy List [[log in to unmask]] on
>behalf of Guy Cox [[log in to unmask]]
>Sent: Thursday, June 05, 2014 12:16 PM
>To: [log in to unmask]
>Subject: Re: Keeping zebrafish embryos in the field of view but letting
>them grow.
>
>Shalin,
>
> I know they do this sort of thing in the Monash
>Micro-Imaging Live Cell course so maybe one of the Steves will reply. My
>own suggestion would to punch a hole just smaller than the FOV in your
>agarose, so the embryo can grow in any direction without you losing track
>of it. A cow-sized hypodermic needle would probably do it. (I've got
>several but I think it would be easier if you sourced them locally).
>
> Guy
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[log in to unmask]]
>On Behalf Of Shalin Mehta
>Sent: Thursday, 5 June 2014 6:15 AM
>To: [log in to unmask]
>Subject: Keeping zebrafish embryos in the field of view but letting them
>grow.
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Hello listers,
>We are imaging zebrafish embryos with 10x and the embryos just about fit
>the field of view at the development stage of interest (4hpf-30hpf).
>
>Are there tried and tested methods for giving the embryos room to grow,
>while keeping them within the field of view?
>
>Embryos are mounted in agarose and we can make space in agarose pad near
>the tail when the embryos are older. But, identifying the growth axis at
>young stages (~4hpf) is difficult.
>
>All inputs appreciated,
>Thanks
>
>Assistant Research Scientist,
>Marine Biological Laboratory,
>7 MBL Street, Woods Hole MA 02543, USA
>
>website: http://mshalin.com
>(office) Lillie 110, (ph) 508-289-7374.
|
