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June 2014

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Confocal Microscopy List <[log in to unmask]>
Subject:
Keeping zebrafish embryos in the field of view but letting them grow.
From:
Shalin Mehta <[log in to unmask]>
Date:
Wed, 4 Jun 2014 16:15:15 -0400
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Confocal Microscopy List <[log in to unmask]>
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Hello listers,
We are imaging zebrafish embryos with 10x and the embryos just about
fit the field of view at the development stage of interest
(4hpf-30hpf).

Are there tried and tested methods for giving the embryos room to
grow, while keeping them within the field of view?

Embryos are mounted in agarose and we can make space in agarose pad
near the tail when the embryos are older. But, identifying the growth
axis at young stages (~4hpf) is difficult.

All inputs appreciated,
Thanks

Assistant Research Scientist,
Marine Biological Laboratory,
7 MBL Street, Woods Hole MA 02543, USA

website: http://mshalin.com
(office) Lillie 110, (ph) 508-289-7374.

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