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The Nikon TiControl software lets you communicate directly with the
chassis. Buried in the menus is a setting for creating an upper Z-position
limit. Once you save the setting it is written to the controller on the
chassis. I don't know if Elements can override this or not, but that's the
first thing I would try. (I'm still using EZC1 on my system so I don't know
what Elements would do)
Craig
On Tue, Jun 17, 2014 at 10:29 AM, Carol Norris <[log in to unmask]>
wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> You can also release the focus lock without moving in z by holding the
> ‘escape’ button in and then pressing the ‘refocus’ button. I try to impress
> upon my users that they should never ‘refocus’ from someone else's
> ‘escaped’ position.
>
> Regards,
> Carol
>
> Carol E. Norris, Ph.D
> Facility Scientist
> Flow Cytometry/Confocal Microscopy Facility
> Biotechnology/Bioservices Center
> University of Connecticut Unit 3149
> 91 N. Eagleville Rd
> Storrs, CT 06269-3149
>
> Phone (860) 486-3080
> Fax (860) 486-5005
>
> On Jun 17, 2014, at 11:46 AM, Sylvie Le Guyader <[log in to unmask]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Another alternative for the xyz positions is to use the NIS element user
> log in. This way the xyz positions are saved for each user and do not show
> up in the other users' account.
> >
> > I also tell users not to tick in the 'move stage to xy position' as this
> easily leads to accidentally moving the stage. Instead we found that if you
> double click in the x or y columns, the stage moves there anyway even if
> the 'move stage' box is unticked. Single click simply selects the line so
> that it can be edited. Accidents are less likely to happen if one needs to
> double click.
> >
> > Med vänlig hälsning / Best regards
> >
> > Sylvie
> >
> > @@@@@@@@@@@@@@@@@@@@@@@@
> > Sylvie Le Guyader
> > Live Cell Imaging Unit Manager
> > Dept of Biosciences and Nutrition
> > Karolinska Institutet
> > Hälsovägen 7
> > Novum, G lift, floor 6
> > 14157 Huddinge
> > Sweden
> > office: +46 (0) 8 5248 1107
> > LCI room 1: +46 (0) 8 5248 1172
> > LCI room 2: +46 (0) 8 5248 3542
> > mobile: +46 (0) 73 733 5008
> >
> >
> >> -----Original Message-----
> >> From: Confocal Microscopy List
> >> [mailto:[log in to unmask]] On Behalf Of Feinstein,
> >> Timothy
> >> Sent: 17 June 2014 17:40
> >> To: [log in to unmask]
> >> Subject: Re: Limiting the z travel range on Nikon Ti
> >>
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Hi Shalin,
> >>
> >> Using the same hardware we have also found the escape button to be
> quite risky
> >> between users. Of equal concern is preserving saved XYZ positions.
> >> We have had to replace expensive bits (but not yet an objective, knock
> on
> >> woodŠ) when the stage automatically tries to return to a previous
> user¹s XYZ
> >> position that was set with a very different stage insert installed.
> >> As a result we ask all users to manually lower the objectives and
> delete their saved
> >> XYZ positions when finished.
> >>
> >> All the best,
> >>
> >>
> >> TF
> >>
> >> Timothy Feinstein, Ph.D. | Confocal Manager
> >> 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
> >> Phone: 616-234-5819 | Email: [log in to unmask]
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >> On 6/17/14, 11:20 AM, "Sylvie Le Guyader" <[log in to unmask]>
> wrote:
> >>
> >>> Hi Shalin
> >>>
> >>> I assume that this happens because one user escapes the objective at
> >>> the end of the imaging session and leaves the system on for the next
> >>> user who then presses the reload button.
> >>> Indeed I do not understand why Nikon has chosen to lock the focus wheel
> >>> when the objective has been escaped so that the only way to get in
> >>> focus again is to reload.
> >>>
> >>> In the meantime I would suggest you do as us: tell users to
> >>> systematically shut down the software and hardware (leaving only the
> >>> gas lasers on) between 2 users. This way when the next user turns the
> >>> system on (it only takes a minute), the objective will be automatically
> >>> escaped and the focus wheel will be unlocked.
> >>>
> >>> Hope that helps :)
> >>>
> >>> Med vänlig hälsning / Best regards
> >>>
> >>> Sylvie
> >>>
> >>> @@@@@@@@@@@@@@@@@@@@@@@@
> >>> Sylvie Le Guyader
> >>> Live Cell Imaging Unit Manager
> >>> Dept of Biosciences and Nutrition
> >>> Karolinska Institutet
> >>> Hälsovägen 7
> >>> Novum, G lift, floor 6
> >>> 14157 Huddinge
> >>> Sweden
> >>> office: +46 (0) 8 5248 1107
> >>> LCI room 1: +46 (0) 8 5248 1172
> >>> LCI room 2: +46 (0) 8 5248 3542
> >>> mobile: +46 (0) 73 733 5008
> >>>
> >>>
> >>>> -----Original Message-----
> >>>> From: Confocal Microscopy List
> >>>> [mailto:[log in to unmask]] On Behalf Of Shalin
> >> Mehta
> >>>> Sent: 17 June 2014 16:47
> >>>> To: [log in to unmask]
> >>>> Subject: Limiting the z travel range on Nikon Ti
> >>>>
> >>>> *****
> >>>> To join, leave or search the confocal microscopy listserv, go to:
> >>>>
> >>>>
> http://scanmail.trustwave.com/?c=129&d=od6g0zsIvVNfDzegQ8BtCuVmNylcDld
> >>>> tMr
> >>>> Os9h-cGA&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-
> >> bin%2fwa%3fA0%3dconfoc
> >>>> alm
> >>>> icroscopy
> >>>> Post images on
> >>>>
> http://scanmail.trustwave.com/?c=129&d=od6g0zsIvVNfDzegQ8BtCuVmNylcDld
> >>>> tMu atpBXISA&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in
> >>>> your posting.
> >>>> *****
> >>>>
> >>>> Hello listers,
> >>>> Our lab has damaged two objectives so far by running them into stage
> >>>> on our inverted Nikon Ti by using the 'Escape' and 'Return' for Z.
> >>>>
> >>>> Our old Nikon (finite tube length) has a mechanical stop on how close
> >>>> the objective can come to the stage and all old objectives are in
> >>>> great shape.
> >>>>
> >>>> Can we program the limits on Z travel on Nikon Ti? Asking our local
> >>>> Nikon folks suggests that the answer is No and we should avoid using
> >>>> escape/return.
> >>>>
> >>>> If this feature is not in the firmware, this is a request to Nikon
> >>>> Japan for such a feature.
> >>>>
> >>>> Thanks
> >>>> Shalin
>
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