*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Excellent point, Craig! Iım running a core facility, so I have a lot of
different users with different applications. So I guess from that
standpoint, Iım looking for thoughts on how the systems compare in range
of use, ease of use, and reliability. Because you are right, each user
will have a different application!
Dr Pamela A. Young
| Light and Optical Microscopist
Australian Centre for Microscopy & Microanalysis
THE UNIVERSITY OF SYDNEY
Rm 116A, Madsen Building F09 | The University of Sydney | NSW | 2006 |
Australia
T +61 2 9351 7527 | F +61 2 9351 7682
E [log in to unmask] | W http://sydney.edu.au/acmm
Incorporating:
Australian Microscopy & Microanalysis Research Facility (AMMRF) | W
http://www.ammrf.org.au <http://www.ammrf.org.au/>
ARC Centre of Excellence for Design in Light Metals | W
http://www.arclightmetals.org.au <http://www.arclightmetals.org.au/>
CRICOS 00026A
This email plus any attachments to it are confidential. Any unauthorised
use is strictly prohibited. If you receive this email in error, please
delete it and any attachments.
On 18/06/2014 12:55 pm, "Craig Brideau" <[log in to unmask]> wrote:
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>You are asking a bit of an 'apples vs. oranges' question here, in that
>different lasers with different accessories achieve different functions.
>Different lasers will be appropriate or inappropriate, depending on the
>type of imaging you want to do and the types of fluorophores you want to
>work with.
>I always start by asking the user what non-linear imaging they want to do.
>The usual answer is 2-photon, but some also want second harmonic
>generation
>capability (SHG), and some want higher-order 3-photon imaging, although
>this is pretty rare. This question gives clues as to what pulse width and
>tuning range the user may require.
>The next is what sort of tissues the user wants to image, and how deep
>they
>want to go. If they want to go very deep, this indicates that longer
>wavelength tuning ranges are appropriate, as well as dispersion control
>with shorter pulse widths, pointing to OPO or just a long-tuning Ti:Saph
>and pulse compression accessories. For relatively shallower imaging on not
>particularly scattering samples, these measures are not necessary.
>Then I ask what sort of fluorophores the user is used to working with, and
>which ones they plan to use. This will help nail down exactly what
>excitation wavelengths will be necessary, indicating what sort of tuning
>range will be necessary out of the laser, and whether or not an OPO will
>be
>needed. For multiple fluorophores it is important to determine if all of
>them can reasonably be excited by a single wavelength, or whether a second
>wavelength would be needed, which again points to an OPO for this
>situation. If the dyes the user wants will all work adequately with a
>single wavelength than just a basic laser is sufficient.
>Finally, the experience level of the user, and whether or not the system
>will be a 'core' system for multiple users, influences how user-friendly
>and turnkey the system and its accessories need to be.
>These are not the only considerations, but I hope it gives you some idea
>of
>the thought processes that go towards selecting a laser.
>
>Craig Brideau
>
>
>On Tue, Jun 17, 2014 at 8:31 PM, Pamela Young <[log in to unmask]>
>wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your
>>posting.
>> *****
>>
>> Hello List,
>>
>> Has anyone done any comparisons of MPM lasers? Most of my experience
>>has
>> been with various versions of the MaiTai and the InSight DeepSee (and
>>of
>> course many much older lasers). So if you have thoughts on how these
>> systems compare to the Chameleon and OPO, I would love your thoughts.
>>
>> Thanks,
>> Pam
>>
>> Dr Pamela A. Young | Light and Optical Microscopist
>> Australian Centre for Microscopy & Microanalysis
>>
>> THE UNIVERSITY OF SYDNEY
>> Rm 116A, Madsen Building F09 | The University of Sydney | NSW | 2006 |
>> Australia
>> T +61 2 9351 7527 | F +61 2 9351 7682
>> E [log in to unmask]<mailto:[log in to unmask]> | W
>> http://sydney.edu.au/acmm
>>
>> Incorporating:
>> Australian Microscopy & Microanalysis Research Facility (AMMRF) | W
>> http://www.ammrf.org.au<http://www.ammrf.org.au/>
>> ARC Centre of Excellence for Design in Light Metals | W
>> http://www.arclightmetals.org.au<http://www.arclightmetals.org.au/>
>>
>> CRICOS 00026A
>> This email plus any attachments to it are confidential. Any unauthorised
>> use is strictly prohibited. If you receive this email in error, please
>> delete it and any attachments.
>>
|
