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Agree, it could be vibration. We saw similar recently (though stripes were
more even), very puzzled until we realised the air table had been switched
off...

cheers,
Rosemary

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
E [log in to unmask]


On 20/06/14 7:51 AM, "Sathya Srinivasan" <[log in to unmask]> wrote:

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>
>Dear Michael,
>As Martin has mentioned, the vibration might be one of the causes. We had
>similar problems with our Nikon A1R recently and one of the legs was
>jammed to the top and was sending vibrations to the images. We were lucky
>to have Craig Brideau and he figured out the problem to be with a jammed
>leg of the anti vibration table. We were also checking the air flow if it
>is pushing in a wind gust to the microscope and we added a temporary
>screen near the vent to rule out that possibility. Our Director came up
>with a suggestion of electrical noise due to faulty grounding which he
>had experienced in his previous lab. Another possibility is the X Y galvo
>itself as we had with our C1si and the X galvo got jammed or was not
>moving properly. Again Craig figured it out of what was happening with
>the XY galvo scan head and we fixed the problem. A simple test if it is
>associated with the table is by tapping the table top or the microscope
>stage and if the lines appears and disappears then it is the table issue.
>The galvo problem will also occur due to the power supply to the
>controller and make sure to rule out that possibility by adding a UPS
>with automatic voltage regulator and surge protector.
> 
>Good luck
> 
>Sathya Srinivasan
>Manager
>RUN Microscopy Facility
>(www.ucalgary.ca/runcore)
>University of Calgary
>Room 126 HMRB
>3330 Hospital Drive, NW
>Calgary, AB, Canada
> 
>> Date: Thu, 19 Jun 2014 12:19:53 -0500
>> From: [log in to unmask]
>> Subject: Re: Olympus FV1000 horizontal lines problem
>> To: [log in to unmask]
>> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>posting.
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>> 
>> Dear Michael--
>> 
>> On 6/19/2014 12:12 PM, Cammer, Michael wrote:
>> > Colleagues have a new Olympus FV1000 with autofocus and are having
>>difficulties with it and asked our advice.  Based on images they sent
>>us, one example posted at
>>https://www.flickr.com/photos/mcammer/14273268488/ , I told them that I
>>thought that the problem of horizontal lines was adjustments made by the
>>autofocus during the scan.  (Another possibility we entertained was
>>laser fluctuation and still another would be sudden temperature change
>>by the incubator unit.)
>> >
>> > Questions:
>> >
>> > 1.       Do you think we have diagnosed the problem correctly?
>> I've seen lines similar to this when focused close to the surface of
>>the 
>> specimen and when the air table isn't damping vibration properly.  Any
>> chance that that's it?
>> 
>> For us, the issue turned out to be a sticky valve on the air table.
>> 
>> Good luck--
>> 
>> Martin Wessendorf
>> 
>> -- 
>> Martin Wessendorf, Ph.D.                   office: (612) 626-0145
>> Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
>> University of Minnesota             Preferred FAX: (612) 624-8118
>> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
>> Minneapolis, MN  55455                    e-mail: [log in to unmask]
>