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Yes, the odor was quite unpleasant. Cargille reformulated their oils. We were able to obtain samples from them for testing. We now routinely use Cargille type HF (Formula code 368) for most of our applications. and Cargille type A (Formula code 1248) for applications where low viscosity is needed. This is primarily for live cell imaging with fast z acquisition where the type HF creates too much drag. Type A is quite runny, and special care is needed on inverted scopes. The Zeiss immersol 518 works fine too. It is a bit less viscous than Cargille HF.

Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109



On Jul 3, 2014, at 8:03 AM, Judy Trogadis wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Hi All
> 
> I would like some advice on immersion oils. I have used Cargille in the past and 
> their LDF type has the correct RI but they claim it has an odor. Has anyone 
> used it and found it unpleasant? They also have type HF but it has a high 
> viscosity (700 cSt) and may be problematic if the cover slip is not attached 
> firmly to the sample. Any comments? I do like the ease of use of their 7.4cc 
> dispensers.
> 
> I have used both Zeiss oil on a Zeiss confocal and Leica oil on a Leica confocal 
> and they have worked well. 
> 
> Any other suggestions?
> 
> Thanks
> 
> 
> Judy Trogadis
> Bio-Imaging Coordinator
> Keenan Research Centre, St. Michael's Hospital
> 209 Victoria Street
> Toronto M5B 1T8, Canada
> office: 416-864-6060 ext. 77612
> imaging facility:           ext. 77434
> cell:        416-254-9330
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