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Hi Ben,
In my experience the critical  point is the sample drifting in the first 20 min after being put on the stage. Then the microscope heating up. We have a combined SIM/PALM system where we changed the cameras to water cooling to prevent this. I would expect temperature fluctuations in the room of plus minus 1 degree to be fine and occasionally our air-conditioning does not manage to cope, but the images are still good. Multi-colour beads in the sample help to align the different channels.
Best wishes
Andreas

-----Original Message-----
From: "Benjamin Abrams" <[log in to unmask]>
Sent: ‎04/‎02/‎2015 19:20
To: "[log in to unmask]" <[log in to unmask]>
Subject: SIM environment requirements

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Hi All,

We are looking in to getting a SIM system and see that the room requirements
are really strict, +/- 0.5 deg C.  This would require significant room
modification (possibly several $100K worth). +/- 1.0 deg C is more doable. 
What is the consensus out there?  How necessary is +/- 0.5?  Can anyone
comment who has experience running their system in a room outside of spec
(even temporarily)? 

Thanks,
Ben