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Hi all, I hope the science is treating you well.
I was wondering if anybody has heard of a good standard sample for
comparing photobleaching. I want to compare how different microscopes
perform under different conditions and wonder how much the sample plays
a role in quantifying the photobleaching rate. I would presumably
calculate the intensity decay over a time lapse as a metric, but wasn't
sure if the sample type was a factor as I don't know much about the
photochemistry. On that note, can anybody recommend a good reference
that discusses the subtleties of photobleaching?
Thanks in advance for your time.
Neil