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Hi Eric,
bear in mind, that the same power settings do not mean the same excitation 
powers. The two objectives have very different diameters of the apertures at
the back focal plane...

Best, zdenek


---------- Původní zpráva ----------
Od: Eric Shelden <[log in to unmask]>
Komu: [log in to unmask]
Datum: 13. 3. 2015 13:21:55
Předmět: Comparing objective lens performance

"*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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All:
I recently had a chance to look at a 40X water immersion 1.1NA Corr lens 
on our Leica SP5 using both conventional and multiphoton illumination. 
Theoretically, it should have given better resolution and better brightness 
than our 20X 0.7 Corr multi-immersion lens. re, with two different 
specimens and preparations it provided only slightly better resolution and 
prequired higher gain settings to achieve saturation with the same laser 
power settings. I was wondering if there was a reasonable explanation, and 
if anyone has had experience with the 40X lens in question.
Thanks,
Eric"