*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear list,

I'd be very grateful for some advice regarding a multiphoton laser for point ablations. We 
want to ablate individual centrosomes in zebrafish embryos without damaging the rest of 
the cell. We plan to do this with water dipping objectives (NA ~1.0) on a confocal system 
with an 80 MHz multiphoton laser. 
What factors should we consider in the choice of laser? 
Is there a benefit for extremely short pulse width (say ~75 fs vs ~140 fs)?
What range of average power or peak power should we be aiming for?
Are there any published reports or guidance for optimising ablations?

Many thanks

Dale

Imaging Facility Manager
UCL Institute of Child Health.