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Hi Gabor,

Olympus also offers CARS commercially, using DeepSea laser (in the US 
and Canada, anyway). Try to have both transmitted and epi detectors - 
both have uses (and you could replce the standard condenser with another 
objective lens, if desired).

http://www.olympus-lifescience.com/en/laser-scanning/fv1200mpe/femtocars/
The femtoCARS equipped FV1200MPE provides a label-free lipid imaging 
capability based on molecular vibrations using the Coherent Anti-Stokes 
Raman Scattering (CARS) microscopy method. In the Olympus design, the 
CARS imaging mode can be combined with two photon excitation 
fluorescence (TPEF) and second harmonic generation (SHG) imaging modes 
as a multimodal femtosecond laser imaging station. CARS is one of a 
number of intrinsic imaging modes requiring no extrinsic label. Because 
it relies on molecular vibrations rather than traditional fluorescence, 
there is no photobleaching.


      Summary

    * Simultaneous Lipid (CH2), TPEF, and SHG imaging. The lipid filter
      set is included and TPEF and SHG filter sets are optional.
    * Uses the standard Olympus DeepSee lasers which are specifically
      tuned to match Olympus optical requirements. No additional laser
      is required.
    * The output of the femtoCARS is pre-set to lipid detection.
    * Standard MPE detection system, up to two forward (transmitted) and
      four backward (epi) Non-Descanned Detection channels.
    * Standard MPE imaging (by-pass CARS add-on) and single photon
      imaging are available.
    * The optical delivery system including a laser-safe enclosure is
      provided and serviced by Olympus Scientific  Solutions Americas
    * The overall footprints for MPE with femtoCARS remain small, but
      different from regular MPE systems.
    * Upright platforms are standard. For inverted platform, please
      contact Olympus Scientific Solutions Americas
    * Acousto-optical modulation (AOM) standard for MPE and manual power
      adjustment operation for the Stokes' beam.
    * Dispersion compensation standard for MPE and for efficient Stokes'
      beam generation.


A couple of CAR reviews

http://www.jlr.org/content/51/11/3091.full
This review reported that CARS (or at least CRS) was first reported by 
a  car company!:

The phenomenon of coherent Raman scattering was first reported by 
scientists at the Ford Motor Co. in 1965 (28 
<http://www.jlr.org/content/51/11/3091.full#ref-28>). Since the 
introduction in the early 1970s (29 
<http://www.jlr.org/content/51/11/3091.full#ref-29>), CARS spectroscopy 
has been widely used as a spectroscopic tool (30 
<http://www.jlr.org/content/51/11/3091.full#ref-30>). The first 
application of CARS to imaging was demonstrated in 1982 with a 
noncollinear beam geometry by scientists at the Naval Research 
Laboratory (31 <http://www.jlr.org/content/51/11/3091.full#ref-31>). 
Nearly two decades later, CARS microscopy was revisited with a collinear 
beam configuration at the Pacific Northwest National Laboratory in 1999 
(32 <http://www.jlr.org/content/51/11/3091.full#ref-32>). Since then, 
the CARS renaissance has stimulated further development and applications 
by researchers from broad scientific disciplines. Continuous 
contributions from many research groups over the last decade have vastly 
improved the design, stability, and sensitivity of CARS microscopy [for 
reviews, see (18 <http://www.jlr.org/content/51/11/3091.full#ref-18>, 23 
<http://www.jlr.org/content/51/11/3091.full#ref-23>--25 
<http://www.jlr.org/content/51/11/3091.full#ref-25>)].


http://www.chem.uci.edu/~potma/AlbaJBO14.pdf
I liked their figure 6, but why did they just go hyperspectral? If you 
go spectral, I suggest branding it super-duper-hyper-multi-spectral 
coherent anti-Stokes Raman super-duper resolution nanoscopy.


best wishes,

George



On 6/23/2015 5:36 AM, Csúcs  Gábor wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> For a user project we would like to establish CARS imaging on one of our scanning confocals (more precisely on a multi-photon system). Unfortunately from the "big fours" (according my information) only Leica offers a commercial solution for CARS but they use a special laser (which for various reasons is not an option for us). Our idea would be to use a "normal" multiphoton laser (SpactraPhysiscs Insight Deepsee with the dual wavelength option) as light source. Ideally we would install this modality on a Zeiss LSM multiphoton system. My question is hence twofold:
> 1) Is there anyone who has established CARS imaging on a Zeiss confocal?
> 2) Is there anyone who has used the above mentioned laser for realizing CARS?
>
> I would very much appreciate any feedback, experience report.
>
> Thanks           Gabor
>
>    


-- 



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42