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Hi, Mike!

My colleague here has some kind of turtle cells in culture.  They may be muscle cells.  Anyway, he says they are very easy to maintain.  You just close up the flask and leave them on the lab bench in the dark.  I believe the reason for "the dark" is that the phenol red in the culture medium generates some free radicals in the light (but I am not sure about this part.)

If you check online, you may be able to find out more about such cells from herps.
Carol

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From: Confocal Microscopy List <[log in to unmask]> on behalf of MODEL, MICHAEL <[log in to unmask]>
Sent: Monday, June 29, 2015 12:22 PM
To: [log in to unmask]
Subject: microscopic samples for schools

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Dear All,

We are planning to introduce some microscopy into schools. We would need a source of cells that would be:
(1) without cell walls (not from an onion)
(2) easy to obtain or maintain
(3) would stick to glass
(4) not too big and not too small (if amoebas are ~0.5 mm that would be too big, red blood cells are probably too small and don't stick to glass)
(5) be alive (cheek cells come out mostly dead, at least from my mouth....)

Maybe C elegans? Or crush some kind of worm and get something out of it? Any ideas? Thank you!

Mike