Subject: | |
From: | |
Reply To: | |
Date: | Mon, 29 Jun 2015 20:12:24 +0200 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Mike,
the usual question comes back to you: What do you want to do with them?
Are you sure cell walls don't work? Moss (no air in the leaves! eg.
https://commons.wikimedia.org/wiki/File:Plagiomnium_affine_laminazellen.jpeg
) or algae from a garden pond are great samples. A sample of the latter
usually will include a bunch of various Protozoa (Paramecium,....) and
small animals that might be what you need. The water from an older vase
of flowers might do too..
I never worked with them, but maybe Dictyostelium would be interesting
for you, if you need something more reproducible. I find Drosophila
cells awkwardly small. Another option: try to find out what your local
zoology department is using for their first year students for teaching
(assuming that "animal kingdom is still taught in practical courses as
it used to be...)
For a school, you probably don't want to use mammalian cells for the
difficulty in cultivation, certainly not human cells for safety reasons.
Steffen
Am 29.06.2015 um 18:22 schrieb MODEL, MICHAEL:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> We are planning to introduce some microscopy into schools. We would need a source of cells that would be:
> (1) without cell walls (not from an onion)
> (2) easy to obtain or maintain
> (3) would stick to glass
> (4) not too big and not too small (if amoebas are ~0.5 mm that would be too big, red blood cells are probably too small and don't stick to glass)
> (5) be alive (cheek cells come out mostly dead, at least from my mouth....)
>
> Maybe C elegans? Or crush some kind of worm and get something out of it? Any ideas? Thank you!
>
> Mike
>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
>
> Marchioninistr. 27
> D-81377 München
> Germany
|
|
|