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Hi Pascal, 

If these are photosynthetic bacteria then their autofluorescence spectra
may help identify them.  In a past life I tried that with dinoflagellates
in a spectrofluorimeter.  I do not think DAPI will be useful unless you
have already ruled out every other possible way of classifying them.

Of course it depends on your experimental design.  If every dish can only
be one out of four or five possible strains then you might get away with
identifying a distinct spectrum for each strain, as long as you show that
spectrum variabilities do not also come from life stage, nutrient
conditions, stress level, ambient  environment etc.  If the list of
possible strains is long or open-ended then you should try something else.
 

Best, 


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology





On 6/29/15, 11:01 AM, "Pascal Weber" <[log in to unmask]> wrote:

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>To join, leave or search the confocal microscopy listserv, go to:
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>
>Infact i have lot of different bacteria and they will not have the same
>spectra. 
>I want to discriminate them only this method. I have not different
>staining just 
>this one but i'm not sure that is the wright way to proceed. My goal is
>to reach 
>a good an simple method to verify if the sample is pure or not. Before to
>do 
>DNA sequencing.