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We measure thickness of live animal cells of any shape in monolayer by dye
exclusion (Microscopy Today
<http://journals.cambridge.org/action/displayJournal?jid=MTO> / Volume 20 /
Issue 03 / May 2012, pp 32-37). It might work on plant cells too though we
have not tried that.

Mike Model

On Wed, Aug 12, 2015 at 1:10 AM, <[log in to unmask]> wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
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>
> Has anyone written a plugin for ImageJ/Fiji that will calculate the
> perpendicular distance between an inner and outer line at various
> positions?
>
> We need to measure the wall thickness on isolated plant cells which, some
> of which look like cross-sections of a cylinder. Now, if they were all
> nearly circular cylinders with even wall thickness, that'd be easy. But
> these cells are far from perfectly cylindrical in shape and the wall
> thickness is uneven.
>
> In the past, we just made 4 measurements of the wall at fixed N-S-E-W
> positions and calculated the average thickness. But with the imaging tools
> available today, it should be possible to write a plugin that will do
> something like this - run a ball along the wall which shrinks and swells
> according to the wall thickness and record the ball diameter at every
> position, for example. That would not only give us the average thickness
> but some useful stats about variability (per cell and per sample) as well.
>
> If anyone knows of something that will do this, you will have our eternal
> gratitude!
>
> thanks,
> Rosemary
>
> Dr Rosemary White
> CSIRO Black Mountain
> GPO Box 1600
> Canberra, ACT 2601
> Australia
>
> T 61 2 6246 5475
> E [log in to unmask]
>