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Hello listers,


I have a couple of questions about condensers for you. I'd like to do some transmitted light imaging in an inverted microscope using high magnification, oil-immersion objectives. For the moment, I don't need to do anything other than brightfield with a high power LED light source. It might be nice to do phase contrast or DIC in the future, but I don't need it now.


My questions are:


1) What are the rules of thumb for matching a brightfield condenser to an objective? I won't be using anything but oil immersion objectives with NA's greater than 1.4.


2) If I do want to do phase contrast or DIC in the future, should I put special consideration into the condenser lens selection now? I imagine the condenser NA will determine what phase contrast rings I can use, but does it impact DIC?


Thanks!

Kyle


Dr. Kyle M. Douglass
Post-doctoral Researcher
EPFL - The Laboratory of Experimental Biophysics
http://leb.epfl.ch/
http://kmdouglass.github.io