On Wed, 25 Aug 1993, Undetermined origin c/o Postmaster wrote:
 
> I have a problem connected to the recent discussion on photobleaching:
> We just want the opposite: a fluid enhancing photobleaching. We are setting
> up a system for doing FRAP in the CLSM, and are testing it with FITC-dextran
> in glycerol - but find this quite hard to bleach.
> Any suggestions?
>
> Uli
>
> ***********************************************
> U. Kubitscheck
> Institut fuer Medizinische Physik und Biophysik
> Robert-Koch-Str. 31
> D-48149 Muenster          <<== NEW / NEU
> Tel. +49 251 83 6935
> Fax  +49 251 83 5121
> ***********************************************
 
Uli-
 
Doug Benson (now at Inovision Corp in Research Triangle Park, North
Carolina) did a study a number of years ago while at Lou Smith's lab at
Baylor college of medicine that showed that photobleaching of FITC is pH
dependent and can actually be used as a good pH indicator.
 
Paul Goodwin
 
P.S. there is a problem with your e-mail address.