I was interested in the suggestion of Alan Hibbs to fade FITC then collect
the Red.  A question: What do you mean by Antifade?  Our concession to
photbleaching has been to use VectaShield mounting medium, which
supposedly inhibits fade through some proprietary process.  What else is
used for antifade?
 Alan Heldman
 Div of Cardiology
Johns Hopkins Univ.
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 On Tue, 21 Sep 1993, Alan Hibbs wrote:
 
> It is possible to use texas red in dual labelling very successfully using the
> BioRad LSM by either using the 488nm or even better the 488 and 514 nm lines
> on the Argon ion laser. The trick is to NOT use antifade in your preparation
> (presuming your FITC fluorescence is good) and to fade out the FITC and then
> collect the texas red image. The texas red is very sub-optimally excited
> with the Argon laser and so it doesn't fade! I have found it works very well
> with dual labelling using antibodies with both live cells and fixed cells.
>