FLUORESCENCE
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                       29 SEPTEMBER - 2 OCTOBER, 1994
            DEPARTMENT OF PHYSIOLOGY, THE UNIVERSITY OF MELBOURNE
 
        The workshop will provide an introduction to the use of fluorescent dyes
as intracellular and extracellular markers. A range of dyes will be used including
those sensitive to ions, electrical potential and cellular proteins as well as m
arkers for intracellular organelles and membrane-bound receptors. The emphasis of
the course will be on signal acquisition and quantitative analysis of probes within
or bound to cells using isolated cells and multicellular preparations.
 
                        Topics to be covered will include:
                        *       Selection and properties of fluorescent probes
                        *       Loading and calibration procedures
                        *       Illuminators and detectors
                        *       Digital image analysis and processing
                        *       Confocal microscopy
                        *       3-dimensional imaging
 
        Commercial vendors will provide equipment and expertise additional to that
available within our laboratories. The number of registrants will be limited according
to the equipment available to ensure adequate time for instruction and experimentation
using a variety of systems, cells and probes.
 
        The registration fee will be $300 ($200 for enrolled students).
 
        We invite anyone interested in attending this workshop to write before April 29 to:
 
Dr. Peter J. Harris,
Department of Physiology,
The University of Melbourne,
PARKVILLE VIC 3052
 
 
Course organisers:
 
Dr. David A Williams                                    Dr. Peter J. Harris