CONFOCALMICROSCOPY Archives

June 1994

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Martin W. Wessendorf
Date:
Mon, 27 Jun 1994 16:44:22 EST
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Martin W. Wessendorf writes:
 
----------------------------Original message----------------------------
In message Paul Robinson writes:
> I have a question which I hope has a simple answer. I have generated some
> nice 2 color images on the MRC 1000. When I combine the two using the
> "merge side" option they look great on the screen, but how do you get them
> off the screen and somewhere useful?
> Biorads CMV PIC conversion utility can't read them, NIH image, optimus and
> Voxelview fail to read them too! COMOS brings them back again with no
> problems. Can some one give me a clue as to why the merged images cause me
> grief? Of course I needed these for a grant due July 1 didn't I!!
 
As I understand it (which, not being a computer jock, I don't), the problem is
that the merged image is 16-bit--most programs are looking for 8-bit or 24-bit
images.  One solution: convert the original unmerged images to tiff format using
NIH Image (or whatever), then merge the two tiff files using Photoshop or some
similar program.  The key is to merge outside of Comos if you want
compatibility.
 
Good Luck!!
 
Martin Wessendorf
--------------------------End of Original Message------------------------------
 
How effective is the merged image with respect to dithering?  We have found
that the output is better if we print the initial merged image and not try
to combine for future manipulation.
 
Tim Noblitt

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