Hello there
 
I am writing a macro for NIH Image to ratio two stacks of confocal data
for quantitative pH imaging.
 
More specifically,  I need to subtract backgrounds (either images by
themselves, but more commonly an offset determined from a part of the
image itself), define an intensity threshold on the two data images,
define a numerical ratio range I want to see, take the ratio and then
clean up the ratio image from pixels outside the ratio range, so to appear
nicer.
 
Before I go too deep into it, is there anyone out there with any comments
or who is aware of anything similar already available, so that I won't
rewrite existing code?
 
 
Thank you,
 
Stamatis Pagakis
University Laboratory of Physiology
University of Oxford, Parks Rd.                FAX:  +44 (0)865 272469
Oxford OX1 3PT,   United Kingdom               Tel:  +44 (0)865 272434