With a slow-scan CCD camera, integrating for a long time, we could just
observe the signal from leciferase/luciferin.  Given the short dwell time
per pixel of the confocal, I would be surprised if it would be a
sufficiently efficient light collecting device to image the weak signal.
 
ScottFraser
 
>Has anyone tried collecting light emission from luciferin using a confocal
>microscope? We are using luciferase as a reporter gene, inserted into
>neurons. The peak emission is at 560 nm, within the range for rhodamine
>detection. We do not actually require an excitation beam, not if the gene
>is turned on.
>
>Judy Trogadis
>Eye Research Institute of Canada and
>University of Toronto
 
 
Scott Fraser  ([log in to unmask])
Caltech, Beckman Institute (139-74)
Pasadena,  CA  91125
818 395-2790 telephone
818 449-5163 fax