CONFOCALMICROSCOPY Archives

March 1995

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Michael Stanley <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sat, 4 Mar 1995 12:16:45 -0600
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Hello All,
        I have a user that is attempting double-label fluroescence for
protein localization in sheep ovaries.  The ovaries seem to auto-fluoresce
very strongly in the FITC channel with some signal in the Rhodamine as
well.  They have talked to other researchers who have not seen this
problem.  The tissue is fixed in Carnoy's and paraffin embedded.
        Has anyone seen or heard of this particular tissue lighting up like
a Christmas Tree?
        TIA,
 
C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia,  MO.    65211
(314) 882-4895
fax= 314-882-0123

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