CONFOCALMICROSCOPY Archives

December 1995

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: How deep can we go?
From:
Margaret Fransen <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 21 Dec 1995 09:00:03 -0500
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A major consideration will be the working distance of your objective lenses.
For example, our 63x lenses have a w.d. of only 90 um-- Beyond that point the
coverslip will flex (we use coverslips supported at the edges), but the image
doesn't change much.
Margaret Fransen
Dept. Anat & Cell Biol, SUNY HSC, Syracuse NY

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