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Date: | Wed, 7 Feb 1996 15:33:44 CST |
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Milton Charlton wrote:
>>>Does anyone have a list of filter wheels which could be software
>>>controlled and which fit the Nikon Optiphot (upright epifluorescence
>>>microscope).
>>>Also I would like to use a light pipe to couple the filter wheel to
>>>the microscope to minimize vibration transfer. Does anyone have
>>>experience with a system using a light pipe?
Kevin Pedley wrote:
>>I use a very nice wheel from a UK company called Cairn Instruments. It
>>steps between adjacent positions in 20 msec, has 6 or 8 filter positions,
>>can be attached to an existing lamphouse or use its own and supply to the
>>microscope is via a light guide. I use it for ratio-imaging from NIH-Image
>>on the Mac and drive it from the modem port via an interface which gives 8
>>TTL output lines, so that I can use the spare lines to drive a shutter,
>>switch media, etc. It also works on the PC.
Michael Herron wrote:
>I am aiming towards setting up a system such as you describe. I would
>appreciate any information you could send me describing your system's details.
>Both hardware and software.
>We would like to visualize calcium fluxes in neutrophils, endothelial cells, and
>keratinocytes. I have priced turnkey systems and balked at the cost. In
>addition I think that a system such as yours might be more flexible.
>I use NIH Image for more common lab duties right now.
I would also recommend the Cairn system. It is a modular system and can be
put together according to your requirements. The light guide is a fluid
filled light guide so it can transmitt UV, so works well with dyes such as
Fura-2. They can be contacted as below:
Dr. Martin Thomas
Cairn Research
email [log in to unmask]
Stephen H. Cody, __ /
Biomedical Confocal Microscopy Research Centre _/ \__/ \
Department of Pharmacology, / \
University of Western Australia, / \
Nedlands WA 6009, \* ____ /
Australia. \_/ \_ _/
email: [log in to unmask] __
Phone: 61 09 346 4569 Fax: 61 09 346 3469 \/
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