CONFOCALMICROSCOPY Archives

February 1996

CONFOCALMICROSCOPY@LISTS.UMN.EDU

Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
From:
Dr M Cannell <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 8 Feb 1996 11:07:48 PST
Content-Type:
text/plain
Parts/Attachments:
text/plain (28 lines)
On Thu, 8 Feb 1996 09:32:37 +0100 Ciro Di Ciro wrote:
 
 
>
> I have a doubt about theorical resolution of my objectives.
>
> For example, I know that it's better use a XY step smaller than the
beam
> diameter of the laser.
> Now, I know that the spot diameter of my 60X is 0.21 mm and the spot
> diameter of the 100X is 0.23  mm.
> My question is: do I have a better resolution with 60X instead of
100X ?
> It is the opposite of that my logic suggest...have some confused
ideas about
> this problem.
>
 
I don't understand what the 'spot sizes' refer to. Assuming you have
diffraction limited performance in your system the resolution depends
only on the numerical aperture of the lens. The higher the numerical
aperture the higher the resolution.
 
Regards
Mark Cannell
 
[log in to unmask]

ATOM RSS1 RSS2