I'm sure you'll get plenty of help on this but here are three of my favorites:
 
Assmus B., P Hutzler, G. Kirchhof, R. Amann, J. R. Lawrence and A.
Hartmann. 1995. In situ localization of Azospirillum brasilense in the
rhizosphere of wheat with fluorescently labeled, rRNA-targeted
oligonucleotide probes and scanning confocal laser microscopy. Appl.
Environ. Microbiol. 61:1013-1019.
 
Lawrence, J.R., D. R. Korber and D. E. Caldwell. 1991. Optical sectioning
of microbial biofilms. J. Bact. 173:6558-6567.
 
Palmer, R.J. Jr. and D. E. Caldwell. 1995. A flowcell for the study of
plaque removal and regrowth.  J. Microbiol. Meth. 24:171-182.
 
I am working with living bacterial biofilms (don't like that fixation
stuff).  If you'd like more info, feel free to call.
 
Rob Palmer
Ctr Env Biotech
423-974-8014
 
>Hello All,
>        As a novice to the area of confocal microscopy, I have been
>searching for some work on bacteria.  Is there anyone out there who is
>working with bacteria using the confocal microscope who would be willing to
>point me in a direction where I would be able to obtain information on
>specimen preparation, (proper fluorescent dyes, etc.), and perhaps some
>references.  I am attempting to generate interest in our department in the
>purchase of a confocal microscope system and one of the parties who has
>shown some interest works with bacteria.  I would be most appreciative of
>any information received.  TIA Sandra Zane
>  Sandra F. Zane, EM Tech.              [log in to unmask]
>  Dept of Biol., UNC Charlotte          (704) 547-4051
>  9201 University City Blvd.            Fax (704) 547-3128
>  Charlotte, NC  28223-0001