In message  <[log in to unmask]> Confocal Microscopy List
writes:
> Dear confocalists:
> Does anyone know or have experience with a fluorescent Nissl stain for
> neuronal tissue?
 
The best options are ethidium bromide (red) and Hoescht 33258 (also known as
bisbenzimide: blue) and Fluoro Nissl Green (green).  The first two were
described first in:     Schmued LC.  Swanson LW.  Sawchenko PE: Some fluorescent
counterstains for neuroanatomical studies.  Journal of Histochemistry &
Cytochemistry.  30(2):123-8, 1982 Feb.
 
For Fluoro Nissl Green, see: Quinn B.  Toga AW.  Motamed S.  Merlic CA: Fluoro
nissl green: a novel fluorescent counterstain for neuroanatomy.  Neuroscience
Letters.  184(3):169-72, 1995 Jan 30.
 
For issues related to autoradiography, see: Schnell SA.  Wessendorf MW:
Bisbenzimide: a fluorescent counterstain for tissue autoradiography.
Histochemistry & Cell Biology.  103(2):111-4, 1995 Feb.
 
Good luck!
 
Martin Wessendorf