If you are willing to swap your secondary, then you could use DCFH for the
mitochondria and Cy3 or Texas Red for the AB. If you are married to your
secondary, then you could try DHE which is red; however, we have had
variable results with it in our hands.
 
________________________________________________________________________________
 
 
Paul Goodwin
Image Analysis Lab
FHCRC, Seattle, WA
 
On Thu, 3 Oct 1996, jane arthur wrote:
 
> Hi.
>
> I've been analyzing SV40-transformed mouse melanoma cells by indirect
> immunofluorescence microscopy using a fluorescein-conjugated secondary
> antibody.  The staining is localised to the nucleus and i would like a good
> counterstain to fluorescein that will enable me to visualise cytoplasmic
> structures in the cell.  Rhodamine has been suggested as a direct
> mitochondrial stain, but to my knowledge this requires living cells and my
> cells have been acetone-fixed.
> Does anyone know of an appropriate, direct fluorescent dye that i can use?
>
> Thank you
>
> Jane Arthur
> [log in to unmask]
>