Paul,  We have developed a program to do exactly that and have published
two papers one in J. Microsc. 173: 115-126, 1994 by Ropysam et al. and
the other in Cytometry 25: 221-234, 1996. For more details contact Badri
Roysam email [log in to unmask]

Jim Turner

> I was wondering if anyone could suggest
a method of counting cells in a
> confocal field of view for use in an FITC-labelled-drug uptake study in
> keratinocytes. I'm finding that I need a method of counting the total number
> of cells, since some cells take up the drug very well and others don't at
> all, so its hard to estimate the proportion of cells fluorescing etc.
>
> I'm now using a two channel system so dual labelling is a possibility (eg
> with Texas Red conjugated to a live cell marker?), but I was hoping that
> there was a simpler solution, particularly since these are time course
> experiments over up to 24 hours.
>
> Thanks in advance!
>
> Paul
>
> Dr Paul White
> Research Officer
> Dermal Therapeutics R&D Programme
> Royal Children's Hospital
> Melbourne, Australia
>
>
> Loretta Donders
> Project Management Assistant
> Dermal Therapeutics R&D Programme
> The Royal Childrens Hospital
> Flemington Road
> Parkville   Vic   3052
> AUSTRALIA
> Phone:  61 3 9345 7909
> Fax: 61 3 9347 7763
> E-Mail:  [log in to unmask]